Effect of Small Intestinal Submucosa on the Protection and Repair of MC₃T₃-E1 Cells Damaged by Dexamethasone-induced Stress


Mian Long


Introduction:The small intestinal submucosa (SIS), a source of allogenic and xenogenic extracellular matrix (ECM), had been playing an increasing role in many biomedical applications. However, there has been little research on how specific interactions between cells and the SIS influence cell protection and repair. In this work, assays of cell viability, function, apoptosis, and migration were used to evaluate the ability of SIS to protect and repair osteoblastic MC3T3-E1 cells from hormone-induced stress damage. Materials & Methods:SIS extracts were prepared with α-MEM basal medium according to ISO10993-12:2007 at the ratio of 3 cm2/mL after incubation for 24 h at 37°C. Cell damage was induced by the addition of dexamethasone (10− 9-10− 5 M for 24 h) to the culture medium, while SIS extracts were added before, along with, or after the dexamethasone treatment. Results & Discussion :Higher concentrations of dexamethasone (10− 7-10− 5 M) caused a reduction in cell viability, cell function, and cell migration but this was markedly reversed by treatment with the SIS extracts. These results indicate that treatment with a SIS extract could provide a valuable method for protecting osteoblastic cells against damage from hormone-induced stress, and for repairing dysfunctional or damaged osteoblastic cells resulting from hormone-induced stress.

Meeting Name

World Congress on Medical Physics and Biomedical Engineering May 26-31, 2012, Beijing, China


Materials Science and Engineering

Keywords and Phrases

Small Intestinal Submocosa; Osteoblasts; Dexamethasone-Induced Stress; Protection; Repair

Document Type

Article - Journal

Document Version


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© 2013 Springer Verlag, All rights reserved.

Publication Date

01 Jan 2013