Preparation and Bioacttve Characteristics of Porous Borate Glass Substrates


Whereas silicate-based bioactive glasses and glass-ceramics have been widely investigated for bone repair or as scaffolds for cell-based bone tissue engineering, recent data have demonstrated that silica-free borate glasses also exhibit bioactive behavior. the objectives of this study were to fabricate porous, three-dimensional substrates of a borate glass and to investigate the biocompatibility of the borate glass substrates by in vitro cell culture with human mesenchymal stem cells (hMSCs) and hMSC-derived osteoblasts (hMSC-Obs). Borate glass particles with sizes 212-355 μm were loosely compacted and then sintered at 600°C to form porous discshaped substrates (porosity ∽ 40%). Partial or nearly complete conversion of the glass substrates to a calcium phosphate (Ca-P) material was achieved by soaking the substrates for 1 day or 7 days in a 0.25 molar K2HPO4 solution at 37°C and at pH of 9.0. Bone marrow derived hMSCs and hMSC-Obs seeded in the samples both adhered to the porous constructs whereas hMSC-Obs markedly synthesized alkaline phosphatase, an early osteogenic marker. These data indicate strong bioactive characteristics for the borate glass constructs and the potential use of the constructs for bone tissue engineering.


Materials Science and Engineering

Keywords and Phrases

Osteoblasts; Phosphatase; Biocompatiile; Engineering; Microscopy

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Document Type

Article - Journal

Document Version


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© 2005 Wiley-Blackwell, All rights reserved.

Publication Date

01 Jan 2005