Bioactivity and Cytocompatibility of Borosilicate Bioglass


Aim: To determinate the bioactivity and cytocompatibility of borosilicate bioglass, and develop a new kind of bioglasses which mainly contain boron as glass network former in the structure.

Methods: The experiment was carried out in the laboratory of School of Materials Science and Engineering of Tongji University from October 2005 to August 2006. By introducing B2O3 into silicate glass (45S5 glass), the borosilicate bioglasses used for bone tissue engineering scaffolds, were prepared at different boron contents (15.4, 30.7, 46.1 mol%). The bioactivity of these borosilicate glasses was evaluated by the formation rate of hydroxyapatite (HAp) crystals with X-ray diffraction method. Taking 45S5 glass as a control, the cytocompatibility to osteoblast cells of these borosilicate glasses was assessed by contact and attachment tests using optical microscope and scanning electron microscope, which were performed at the Biomaterials Research Group at the University of Missouri-Rolla.


  1. Bioactivity: The formation rate of HAp on the samples increased with the increasing of boron content, so did the crystallinity of HAp. The bioactivity improved with increasing of boron content.
  2. Cytocompatibility: Comparing with 45S5 bioglass, the borosilicate glass with high content boron did have certain inhibition on the growth of osteoblast cells, but osteoblast cells which attached on the borosilicate glass plate surface or the ones near the interface of glass plate still presented good growth on the whole.

Conclusion: Borosilicate bioglasses may serve as a promising scaffold material for bone tissue engineering. They not only have great bioactivity, but also have a good cytocompatibility to osteoblast cells.


Materials Science and Engineering

Second Department

Nuclear Engineering and Radiation Science

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Document Type

Article - Journal

Document Version


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© 2007 Journal of Clinical Rehabilitative Tissue Engineering Research, All rights reserved.

Publication Date

01 Sep 2007