Keywords and Phrases
eDNA metabarcoding; environmental DNA; fish; freshwater; Ozark streams; survey
Environmental DNA (eDNA) sampling provides a method for assessing fish communities that has potential as a supplement to traditional sampling methods due to its ability to save time as well as its non-invasive nature. This is a method in which from just one sample, eDNA from multiple individual species are able to be sequenced in tandem and the resulting reads identified to describe a community. In order to evaluate this technique and its efficacy for monitoring fish community diversity, we collected water samples alongside surveys performed by the Missouri Department of Conservation sampling program in summers 2020-21. DNA were extracted from these samples and amplified via polymerase chain reaction (PCR), using several targeted mitochondrial gene markers, which were then cleaned and sequenced. We investigated the variation in species detection among different gene markers. We also sought to determine environmental factors involved in variation of eDNA results between summer and winter. We compared the species detected by eDNA to traditional survey detection. Using a variety of statistics including NMDS, ANOSIM, UPGMA clustering, and others, we provide support for the implementation of eDNA metabarcoding techniques to supplement traditional sampling as a robust technique able to provide optimal coverage of fish communities in the Ozarks. We found an average detection rate of 2 species identified by eDNA metabarcoding for every 1 identified by traditional methods--Abstract, page iii.
Duvernell, David D. (David Douglas), 1970-
Frank, Ronald L.
Olbricht, Gayla R.
M.S. in Applied and Environmental Biology
Missouri University of Science and Technology
x, 69 pages
© 2022 Veronica Marian Lee, All rights reserved.
Thesis - Open Access
Electronic OCLC #
Lee, Veronice Marian, "Validation of eDNA metabarcoding : A comparison to traditional survey methods in Ozark streams" (2022). Masters Theses. 8108.