"Oxidative stress plays an important role in neurodegenerative disorders such as Parkinson's disease and Alzheimer's disease. Methamphetamine (METH) is an amphetamine analog that causes degeneration of the dopaminergic system in mammals and subsequent oxidative stress. In our present study, we have used immortalized human brain microvascular endothelial cells to test whether METH induces oxidative stress in vitro and the ability of a new antioxidant, N-Acetylcysteine amide (NACA), to prevent METH-induced oxidative stress. Our studies show that NACA protects against METH- induced oxidative stress in immortalized human brain microvascular endothelial cells. NACA significantly protected the integrity of our Blood Brain Barrier (BBB) model, as shown by permeability and trans-endothelial electrical resistance (TEER) studies. NACA also significantly increased the levels of intracellular reduced glutathione (GSH), and glutathione peroxidase (GPx). Lipid peroxidation indicator, malondialdehyde (MDA), increased dramatically after METH exposure, but this increase was almost completely prevented when the cells were also treated with NACA. Reactive oxygen species (ROS) generation also increased after METH exposure, but was reduced to control levels with NACA treatment, as measured by dichlorofluorescin (DCF). These results suggest that NACA protects BBB integrity, which could prevent oxidative stress in vitro, and the effectiveness of this antioxidant should be evaluated for the treatment of neurodegenerative diseases in the future"--Abstract, page iii.
M.S. in Chemistry
Missouri University of Science and Technology
ix, 52 pages
© 2008 Xinsheng Zhang, All rights reserved.
Thesis - Restricted Access
Methamphetamine -- Physiological effect
Nervous system -- Degeneration -- Pathophysiology
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Electronic access to the full-text of this document is restricted to Missouri S&T users. Otherwise, request this publication directly from Missouri S&T Library or contact your local library.http://merlin.lib.umsystem.edu/record=b6665295~S5
Zhang, Xinsheng, "N-Acetylcystein amide protects against methamphetamine-induced oxidative stress in human brain endothelial cells" (2008). Masters Theses. 81.
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