Masters Theses
Abstract
"The entire time course of the hydrolysis of p-nitrophenyl-a-D-glucoside by the enzyme glucoamylase I was followed spectrophotometrically. Using time versus absorbance data the constants in the Michaelis-Menten equation were determined. The effect of the addition of product to the initial reaction mixture and the effect of solvent and temperature were determined. It was found that product produced during the reaction did not have the same effect upon the rate as did product added initially to the reaction mixture. The presence of substrate caused the enzyme to acquire protection against inhibition by the product"-- Abstract, p. ii
Advisor(s)
Poling, Bruce E.
Committee Member(s)
Crosser, Orrin K.
Siehr, Donald J.
Department(s)
Chemical and Biochemical Engineering
Degree Name
M.S. in Chemical Engineering
Publisher
University of Missouri--Rolla
Publication Date
1975
Pagination
vi, 40 pages
Note about bibliography
Includes bibliographical references (pages 36-37)
Rights
© 1975 Mark L. Dickhaus, All rights reserved.
Document Type
Thesis - Open Access
File Type
text
Language
English
Thesis Number
T 4059
Print OCLC #
5984420
Recommended Citation
Dickhaus, Mark L., "A study of product inhibition in the reaction of p-NITROPHENYL- α - D - GLUCOSIDE catalyzed by the enzyme glucoamylase I." (1975). Masters Theses. 3033.
https://scholarsmine.mst.edu/masters_theses/3033