Doctoral Dissertations

Keywords and Phrases

Bioanalysis

Abstract

"Despite continuous effort and progress in cancer detection and therapy, cancer remains one of our greatest health concerns because of its low survival rate and rapid incidence increase. One reason for this is the late detection and therefore pre-cancer diagnosis is crucial. In pre-cancer studies, cancer biomarkers become significant because of the useful information it contains such as possible cancer type and stage. Separation and detection technique plays an important role in cancer biomarker identification.

Coenzyme A (CoA) facilitates more than 100 chemical reactions in cells. Because of its low abundance accumulated in cells, it is critical to develop a sensitive method to detect CoA compounds in biological samples in order to study it as a cofactor.

The valuable characteristics of capillary electrophoresis (CE), such as rapid analysis, high separation efficiency and minimal consumption of sample and buffer solutions, make it a unique analytical technique. The combination of the high specificity and sensitivity of tandem mass spectrometry (MS/MS) with the high separation ability of high performance liquid chromatography (HPLC) facilitates the sensitive and specific analysis in complex matrices like urine and blood. In this body of work, new methods were developed using CE-UV, CE-LIF and HPLC-MS/MS to determine modified nucleosides, CoAs, and sarcosine, proline, kynurenine, uracil and glycerol-3-phosphate. These methods were applied to measure the above mentioned analytes in either urine samples or cell extracts and proved to be simple, fast, reliable and powerful. Based on these newly developed methods, cancer biomarker screening is undertaken and new biomarkers will be identified"--Abstract, page iv.

Advisor(s)

Ma, Yinfa

Committee Member(s)

Whitefield, Philip D.
Ercal, Nuran
Nam, Paul Ki-souk
Brown, Roger F.

Department(s)

Chemistry

Degree Name

Ph. D. in Chemistry

Sponsor(s)

  • Iowa State University. Plant Sciences Institute
  • Missouri University of Science and Technology. Environmental Research Center
  • Missouri University of Science and Technology. Department of Chemistry

Publisher

Missouri University of Science and Technology

Publication Date

2012

Pagination

xi, 88 pages

Note about bibliography

Includes bibliographical references (pages 77-87).

Rights

© 2012 Yongqing Jiang, All rights reserved.

Document Type

Dissertation - Open Access

File Type

text

Language

English

Subject Headings

Cancer -- Diagnosis -- Research
Biochemical markers
Capillary electrophoresis
High performance liquid chromatography

Thesis Number

T 10166

Print OCLC #

858812176

Electronic OCLC #

858812940

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