Keywords and Phrases
"Phenylalanine ammonia-lyase (PAL) (EC 4. 3. 1. 5) activity was greatly increased in Schizophyllum commune 4-40 by growing the organism in a medium containing L-Phenylalanine. S. commune 4-40 PAL was purified 19.2 fold by a procedure utilizing Sephadex G-50 chromatography, ammonium sulfate fractionation and chromatographic separation on Sephadex G-200. The enzyme has a native molecular weight of 247,000. The Km value for L-phenylalanine is 0.232 mM, the Vmax is 0.415 mM/minute and the kcat is 0.0164/second for the purified lyase.
Two attempts were made to synthesize a cDNA library from mRNA isolated from S. commune 4-40. Soybean PAL DNA and Rhodotorula rubra PAL DNAs were used as probes for screening for the PAL gene in the cDNA libraries and a genomic library prepared by Specht and Ulrich. The inability to cut the DNA isolated from reconstituted phage supposedly containing DNA from the cDNA library into more than 2 fragments with a mixture of two restriction endonucleases plus the high incidence of positive plaques in the screening tests, suggest that a true library had not been synthesized"--Abstract, page iii.
Siehr, Donald J.
Hanna, Samir B.
Frank, Ronald L.
Ph. D. in Chemistry
University of Missouri--Rolla
x, 111 pages
© 1993 Jiamin Zeng, All rights reserved.
Dissertation - Restricted Access
DNA -- Analysis
Print OCLC #
Electronic OCLC #
Link to Catalog Record
Electronic access to the full-text of this document is restricted to Missouri S&T users. Otherwise, request this publication directly from Missouri S&T Library or contact your local library.http://merlin.lib.umsystem.edu/record=b2668576~S5
Zeng, Jiamin, "The isolation and characterization of phenylalanine ammonia lyase from Schizophyllum commune and an attempt to isolate the gene" (1993). Doctoral Dissertations. 1019.
Share My Dissertation If you are the author of this work and would like to grant permission to make it openly accessible to all, please click the button above.