Natural and Synthetic Hormone Removal Using the Horseradish Peroxidase Enzyme: Temperature and pH Effects


The primary objective of our research was to establish the technical feasibility of using the horseradish peroxidase (HRP) enzyme for natural and synthetic estrogens-estrone (E1), 17β-estradiol (E2), estriol (E3), and 17α-ethinylestradiol (EE2)-removal. the effects of temperature and pH on enzymatic treatment kinetics were investigated. Residual estrogen concentrations were quantified by liquid chromatography, coupled with mass spectrometry analysis. in a synthetic solution at pH 7 and 25±1 °C, the HRP enzyme-catalyzed process was capable of achieving 92-100% removal of E1, E2, E3, and EE2 within 1 h of treatment with an HRP activity of 0.017 U/ml. the influence of the pH (5-9) and temperature (5-35 °C) on estrogen removal was observed to be significant, with the optimum pH near neutral conditions. the results also showed that wastewater constituents significantly impact the HRP-catalyzed estrogen removal. the experimental research proved that the HRP-catalyzed system is technically feasible for the removal of the main estrogens present in the environment at low concentrations.


Civil, Architectural and Environmental Engineering

Keywords and Phrases

Enzyme; Estrogens; HRP; Kinetic; Wastewater

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Document Type

Article - Journal

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© 2006 Elsevier, All rights reserved.

Publication Date

01 Jan 2006