Fabrication of Culture-Based Biochips for Detecting Microorganisms in Environmental Samples


In this paper, we describe a culture-based biochip for detecting microorganisms in environmental samples in an improved, timesaving, and cost-efficient manner. The biochips use the paraffinophillic nature of microorganisms to rapidly detect and differentiate them in sewage samples collected from wastewater treatment plants. A new method of depositing paraffin was developed to fabricate the biochips. The developed technique uses butanol solvent to keep paraffin liquid during spin-coating and thick photoresist for patterning. Using this approach, paraffin layers of 5-70 µm have been successfully fabricated and patterned. These biochips were used to demonstrate rapid, 5-min detection of microorganisms responsible for reactor foaming in wastewater treatment.

Meeting Name

IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) (2002: Oct. 23-26, Houston, TX)


Civil, Architectural and Environmental Engineering

Keywords and Phrases

Activated sludge process; Biotechnology; Cell culture; Foams; Molecular biology; Paraffins; Photoresists; Sewage analysis; Spin coating; Biochips; Mycobacteria; Nocardiafoam; Paraffinophilic nature; Microorganisms

International Standard Serial Number (ISSN)


Document Type

Article - Conference proceedings

Document Version


File Type





© 2002 Institute of Electrical and Electronics Engineers Inc. (IEEE) , All rights reserved.

Publication Date

01 Oct 2002