Developing Culture-Based Biochips for Detecting Microorganisms in Wastewater Treatment


In this paper, we describe a culture-based biochip for detecting microorganisms in environmental samples in an improved, timesaving, and cost-efficient manner. The biochips use the paraffinophilic nature of microorganisms to rapidly detect and differentiate them in sewage samples collected from wastewater treatment plants. A new method of depositing paraffin was developed in order to fabricate such biochips. The developed technique uses butanol solvent to keep paraffin liquid during deposition by spin-coating and thick photoresist for patterning. Using this approach, 18 mm glass biochips with paraffin layers of 5-70 µm have been successfully fabricated. These biochips were used to demonstrate rapid, 5-min detection of microorganisms responsible for reactor foaming in wastewater treatment.

Meeting Name

2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology (2002: May 2-4, Madison, WI)


Civil, Architectural and Environmental Engineering

Keywords and Phrases

Bioassay; Biochips; Microarrays; Microorganisms; Photoresists; Plants (botany); Reclamation; Sewage; Sewage treatment plants; Cost-efficient; Environmental sample; Glass biochips; mycobacteria; Thick photoresists; Wastewater treatment plants; Wastewater treatment; mycobacteria; paraffin

International Standard Book Number (ISBN)


Document Type

Article - Conference proceedings

Document Version


File Type





© 2002 Institute of Electrical and Electronics Engineers (IEEE) , All rights reserved.

Publication Date

01 May 2002