Determination of Total Polyamines in Tumor Cells by High-Performance Capillary Zone Electrophoresis with Indirect Photometric Detection


Tumor cells contain much higher levels of common poly amines such as putrescine, spermidine, and spermine than normal cells. Our previous work demonstrated that high-performance capillary zone electrophoresis with Indirect photometric detection was suitable for the determination of underlvatized free polyamines in tumor cells. We now report Improved sensitivity (7-fold), greater quantitative reproducibility, and quantitation of both bound and free, underlvatized polyamines in PC 12 tumor cells. 1,7-Diaminoheptane was used as an internal standard for quantification. Femtomoles of three polyamines and simple cations such as K+ and Na+ were easily separated in less than 10 min. The quantitations for total spermidine and spermine (bound and free) were found to be 2-fold higher than the quantitation for free, unbound polyamines using our previous method. The signal response was linear over 2 orders of magnitude. The method described here provides a fast and simple technique for quantitation of bound, unbound, and total polyamines for use in the study of the biological role of polyamines.



Keywords and Phrases

1,7 Diaminoheptane; 1,7-diaminoheptane; Diamine; Polyamine; Putrescine; Spermidine; Spermine; Adrenal Tumor; Animal; Cell Strain; Chemistry; Comparative Study; Methodology; Pheochromocytoma; Rat; Adrenal Gland Neoplasms; Comparative Study; Electrophoresis; PC12 Cells; Putrescine; Support, Non-U.S. Gov't

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Article - Journal

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© 1993 American Chemical Society (ACS), All rights reserved.

Publication Date

01 Mar 1993