Abstract

A statistical thermodynamic theory of chiral solute retention and separation using chiral stationary phase high-performance liquid chromatography (CSP-HPLC) is formulated and applied in the limit of infinite dilution of solute to models of chemically bonded CSPs consisting of either chiral tetrahedral moieties or chiral molecular cavities intended to simulate a cyclodextrin CSP. Explicit expressions are derived for the corresponding separation factors and the likelihood of enantiomeric separation is investigated as a function of the multitude of possible interactions and retention modes for the enantiomers and CSPs considered. The separation factor reduces to a simple Boltzmann factor whenever a dominant retention mode, usually involving strong hydrogen bonding, prevails for both enantiomers. In mixed mobile phases, inversion of enantiomer elution order as a function of composition is a predicted possibility with a cyclodextrin CSP. Enantiomeric separation by diffusion through aqueous solution interphases of cyclodextrin is also investigated. © 1988, American Chemical Society. All rights reserved.

Department(s)

Chemistry

Comments

National Institute of General Medical Sciences, Grant R01GM036292

International Standard Serial Number (ISSN)

1520-6882; 0003-2700

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2023 American Chemical Society, All rights reserved.

Publication Date

01 Mar 1988

PubMed ID

3377166

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