Determination of F₂-isoprostanes in Cultured Human Lung Epithelial Cells after Exposure to Metal Oxide and Silica Nanoparticles by High-Performance Liquid Chromatography/Tandem Mass Spectrometry


The environmental impact of nanotechnology has caused a great concern. Many in vitro studies showed that many types of nanoparticles were cytotoxic. However, whether these nanoparticles caused cell membrane damage was not well studied. F2-isoprostanes are specific products of arachidonic acid peroxidation by nonenzymatic reactive oxygen species and are considered as reliable biomarkers of oxidative stress and lipid peroxidation. In this article, we investigated the cytotoxicity of different nanoparticles and the degree of cellular membrane damage by using F2-isoprostanes as biomarkers after exposure to nanoparticles. The human lung epithelial cell line A549 was exposed to four silica and metal oxide nanoparticles: SiO2 (15 nm), CeO2 (20 nm), Fe2O3 (30 nm), and ZnO (70 nm). The levels of F2-isoprostanes were determined by using highperformance liquid chromatography/mass spectrometry. The F2-isoprostanes' peak was identified by retention time and molecular ion m/z at 353. Oasis HLB cartridge was used to extract F2-isoprostanes from cell medium. The results showed that SiO2, CeO2, and ZnO nanoparticles increased F2-isoprostanes levels significantly in A549 cells. Fe2O3 nanoparticle also increased F2-isoprostanes level, but was not significant. This implied that SiO2, CeO2, ZnO, and Fe2O3 nanoparticles can cause cell membrane damage due to the lipid peroxidation. To the best of our knowledge, this is the first report on the investigation of effects of cellular exposure to metal oxide and silica nanoparticles on the cellular F2-isoprostanes levels.



Keywords and Phrases

A549 cells; Arachidonic acids; Cellular membranes; Cytotoxic; F2-isoprostanes; Human lung; In-vitro; Isoprostanes; Lipid peroxidation; Liquid chromatography/mass spectrometry; Liquid chromatography/tandem mass spectrometry; Metal oxide nanoparticles; Metal oxides; Molecular ions; Non-enzymatic; Peroxidation; Reactive oxygen species; Retention time; Silica nanoparticles; ZnO; ZnO nanoparticles; Biological organs; Cell culture; Cell membranes; Environmental impact; Liquids; Mass spectrometry; Membranes; Metallic compounds; Oxidation; Oxidative stress; Oxygen; Projectiles; Silica; Zinc oxide; Nanoparticles; biochemistry; biomarker; enzyme activity; functional morphology; lipid; liquid chromatography; mass spectrometry; particle size; F2-isoprostanes

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© 2010 Taylor & Francis Ltd., All rights reserved.

Publication Date

01 May 2010