The Effect of Photoinitiators on Intracellular AKT Signaling Pathway in Tissue Engineering Application


Free-radical photopolymerization initiated by photoinitiators is an important method to make tissue engineering scaffolds. To advance understanding of photoinitiator cytocompatibility, we examined three photoinitiators including 2,2-dimethoxy-2-phenylacetophenone (DMPA), Irgacure 2959 (I-2959), and eosin Y photoinitiating system (EY) in terms of their effects on the viability of HN4 cells and expression levels of intracellular AKT and its phosphorylated form p-AKT. Our results show that the photoinitiators and their UV-exposed counterparts affect intracellular AKT signaling, which can be used in conjunction with cell viability for cytocompatibility assessment of photoinitiators.


Chemical and Biochemical Engineering

Keywords and Phrases

Free radicals; Photopolymerization; Scaffolds (biology); Tissue; Tissue engineering, Cytocompatibility; Expression levels; Free radical photopolymerization; Photo-initiator; Photoinitiating systems; Signaling pathways; Tissue engineering applications; Tissue engineering scaffold, Cell signaling, photoinitiator; protein kinase B; 2,2-dimethoxy-2-phenylacetophenone; acetophenone derivative; protein kinase B, Article; cell proliferation assay; cell viability; controlled study; human; human cell; hydrogel; light exposure; oxidative stress; polymerization; priority journal; protein expression; tissue engineering; Western blotting; WST-1 assay; cell survival; chemistry; drug effects; metabolism; photochemistry; procedures; signal transduction; tissue engineering; tissue scaffold; ultraviolet radiation, Acetophenones; Cell Survival; Photochemistry; Polymerization; Proto-Oncogene Proteins c-akt; Signal Transduction; Tissue Engineering; Tissue Scaffolds; Ultraviolet Rays

International Standard Serial Number (ISSN)

2047-4830; 2047-4849

Document Type

Article - Journal

Document Version


File Type





© 2015 Royal Society of Chemistry, All rights reserved.

Publication Date

01 Feb 2015

PubMed ID