The Effect of Photoinitiators on Intracellular AKT Signaling Pathway in Tissue Engineering Application
Free-radical photopolymerization initiated by photoinitiators is an important method to make tissue engineering scaffolds. To advance understanding of photoinitiator cytocompatibility, we examined three photoinitiators including 2,2-dimethoxy-2-phenylacetophenone (DMPA), Irgacure 2959 (I-2959), and eosin Y photoinitiating system (EY) in terms of their effects on the viability of HN4 cells and expression levels of intracellular AKT and its phosphorylated form p-AKT. Our results show that the photoinitiators and their UV-exposed counterparts affect intracellular AKT signaling, which can be used in conjunction with cell viability for cytocompatibility assessment of photoinitiators.
L. Xu et al., "The Effect of Photoinitiators on Intracellular AKT Signaling Pathway in Tissue Engineering Application," Biomaterials Science, vol. 3, no. 2, pp. 250-255, Royal Society of Chemistry, Feb 2015.
The definitive version is available at https://doi.org/10.1039/c4bm00245h
Chemical and Biochemical Engineering
Keywords and Phrases
Free radicals; Photopolymerization; Scaffolds (biology); Tissue; Tissue engineering, Cytocompatibility; Expression levels; Free radical photopolymerization; Photo-initiator; Photoinitiating systems; Signaling pathways; Tissue engineering applications; Tissue engineering scaffold, Cell signaling, photoinitiator; protein kinase B; 2,2-dimethoxy-2-phenylacetophenone; acetophenone derivative; protein kinase B, Article; cell proliferation assay; cell viability; controlled study; human; human cell; hydrogel; light exposure; oxidative stress; polymerization; priority journal; protein expression; tissue engineering; Western blotting; WST-1 assay; cell survival; chemistry; drug effects; metabolism; photochemistry; procedures; signal transduction; tissue engineering; tissue scaffold; ultraviolet radiation, Acetophenones; Cell Survival; Photochemistry; Polymerization; Proto-Oncogene Proteins c-akt; Signal Transduction; Tissue Engineering; Tissue Scaffolds; Ultraviolet Rays
International Standard Serial Number (ISSN)
Article - Journal
© 2015 Royal Society of Chemistry, All rights reserved.
01 Feb 2015