Title

Folate-Decorated Polyamidoamine Dendrimer Nanoparticles for Head and Neck Cancer Gene Therapy

Abstract

Gene delivery systems have been developed on the basis of dendrimers and many other types of nanoparticle carriers, but few have been developed for head and neck squamous cell carcinomas (HNSCC). Herein, we describe the design and synthesis of fluorescently labeled, folic acid-decorated polyamidoamine (PAMAM) generation 4 (G4) dendrimer conjugates for HNSCC-targeted gene delivery. This delivery system comprises a dendrimer as the carrier that is conjugated with folic acid (FA) as HNSCC targeting moiety and imaging agents fluorescein isothiocyanate (FITC) or IRDye 800CW (NIR) for in vitro trafficking or bioimaging, respectively. By complexing with plasmid or siRNA, G4-FA/plasmid (or siRNA) significantly enhances gene transfection or knockdown efficiency in HNSCC cells. In a mouse xenograft model of HNSCC, this versatile G4-FA vector shows high biocompatibility, tumor targeting, high uptake, and sustained retention, making it a suitable platform for HNSCC gene therapy.

Department(s)

Chemical and Biochemical Engineering

Comments

Part of the Methods in Molecular Biology book series (MIMB, volume 1974)

Keywords and Phrases

Dendrimer; Folate receptor; Gene therapy; Head and neck cancer; Targeted delivery

International Standard Serial Number (ISSN)

1064-3745

Document Type

Book - Chapter

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2019 Springer Science+Business Media, LLC, All rights reserved.

Publication Date

01 May 2019

PubMed ID

31099016

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