High Efficiency Separation of Microbial Aggregates Using Capillary Electrophoresis

Abstract

Recent advances in the technique of capillary electrophoresis have demonstrated fast, highly efficient separation of mixtures of intact microbes. This paper describes the application of this technique for the separation of microbial aggregates of Micrococcus luteus, Saccharomyces cerevisiae, or Alcaligenes faecalis. The aggregates of these microbes were resolved into several highly efficient peaks with analysis times under 10 min and efficiencies approaching 1 000 000 plates m-1 in some cases. A reproducible relationship was found between the electrophoretic mobility and the aggregation number or size of the cluster under a given set of experimental conditions. Often, cellular aggregation was reversible with brief immersion in an ultrasound bath. This reversibility was confirmed by visual microscopy and electrophoretic data.

Department(s)

Biological Sciences

Second Department

Chemistry

Keywords and Phrases

Yeast; Bacterium; Capillary Electrophoresis Characterization; Polymer; Separation

International Standard Serial Number (ISSN)

0378-1097;1574-6968

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2000 John Wiley & Sons, All rights reserved.

Publication Date

01 Aug 2000

PubMed ID

10913863

Link to Full Text

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