Proliferation and Function of MC3T3-E1 Cells on Freeze-cast Hydroxyapatite Scaffolds with Oriented Pore Architectures

Abstract

Previous work by the authors showed that hydroxyapatite (HA) scaffolds with different types of oriented microstructures and a unique 'elastic-plastic' mechanical response could be prepared by unidirectional freezing of suspensions. The objective of the present work was to evaluate the in vitro cellular response to these freezecast HA scaffolds. Unidirectional scaffolds with approximately the same porosity (65-70%) but different pore architectures, described as 'lamellar' (pore width = 25 ± 5 lm) and 'cellular' (pore diameter = 100 ± 10 lm), were evaluated. Whereas both groups of scaffolds showed excellent ability to support the proliferation of MC3T3-E1 pre-osteoblastic cells on their surfaces, scaffolds with the cellular-type microstructure showed far better ability to support cell proliferation into the pores and cell function. These results indicate that freeze-cast HA scaffolds with the cellular-type microstructure have better potential for bone repair applications.

Department(s)

Biological Sciences

Second Department

Materials Science and Engineering

Keywords and Phrases

Hydroxyapatite

International Standard Serial Number (ISSN)

0957-4530

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2009 Springer Verlag, All rights reserved.

Publication Date

01 May 2009

Link to Full Text

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