Staining of the Actin Cytoskeleton during Cell Division in Budding Yeast and Mammalian Cells
Observation of actin at the cortex in dividing cells can be accomplished using the fungal toxin phalloidin conjugated to fluorophores. Protocols for staining both budding yeast and cultured mammalian cells with fluorescent phalloidin are described. This technique can be combined with immunofluorescence to image actin filaments and other proteins involved in cell division simultaneously.
K. Shannon, "Staining of the Actin Cytoskeleton during Cell Division in Budding Yeast and Mammalian Cells," Mitosis: Methods and Protocols, vol. 2415, pp. 87 - 94, Springer, Jan 2022.
The definitive version is available at https://doi.org/10.1007/978-1-0716-1904-9_6
Keywords and Phrases
Actin; Cytokinesis; Fixation; Fluorescence microscopy; Formaldehyde; Phalloidin
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01 Jan 2022