Staining of the Actin Cytoskeleton during Cell Division in Budding Yeast and Mammalian Cells

Author

Katie Shannon, Missouri University of Science and TechnologyFollow

Abstract

Observation of actin at the cortex in dividing cells can be accomplished using the fungal toxin phalloidin conjugated to fluorophores. Protocols for staining both budding yeast and cultured mammalian cells with fluorescent phalloidin are described. This technique can be combined with immunofluorescence to image actin filaments and other proteins involved in cell division simultaneously.

Recommended Citation

K. Shannon, "Staining of the Actin Cytoskeleton during Cell Division in Budding Yeast and Mammalian Cells," Mitosis: Methods and Protocols, vol. 2415, pp. 87 - 94, Springer, Jan 2022.

The definitive version is available at https://doi.org/10.1007/978-1-0716-1904-9_6

Department(s)

Biological Sciences

Keywords and Phrases

Actin; Cytokinesis; Fixation; Fluorescence microscopy; Formaldehyde; Phalloidin

International Standard Serial Number (ISSN)

1940-6029; 1064-3745

Document Type

Book - Chapter

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2022 Springer, All rights reserved.

Publication Date

01 Jan 2022