Three-dimensional (3D) bioprinting technologies have shown great potential in the fabrication of 3D models for different human tissues. Stem cells are an attractive cell source in tissue engineering as they can be directed by material and environmental cues to differentiate into multiple cell types for tissue repair and regeneration. In this study, we investigate the viability of human adipose-derived mesenchymal stem cells (ASCs) in alginate-gelatin (Alg-Gel) hydrogel bioprinted with or without bioactive glass. Highly angiogenic borate bioactive glass (13-93B3) in 50 wt% is added to polycaprolactone (PCL) to fabricate scaffolds using a solvent-based extrusion 3D bioprinting technique. The fabricated scaffolds with 12 x 12 x 1 mm3 in overall dimensions are physically characterized, and the glass dissolution from PCL/glass composite over a period of 28 days is studied. Alg-Gel composite hydrogel is used as a bioink to suspend ASCs, and scaffolds are then bioprinted in different configurations: Bioink only, PCL+bioink, and PCL/glass+bioink, to investigate ASC viability. The results indicate the feasibility of the solvent-based bioprinting process to fabricate 3D cellularized scaffolds with more than 80% viability on day 0. The decrease in viability after 7 days due to glass concentration and static culture conditions is discussed. The feasibility of modifying Alg-Gel with 13-93B3 glass for bioprinting is also investigated, and the results are discussed.


Biological Sciences

Second Department

Materials Science and Engineering

Third Department

Mechanical and Aerospace Engineering


The glass used in this study was provided by MOSCI Corporation, Rolla, MO, USA.

Keywords and Phrases

Alginate-gelatin bioink; Bioprinting; Borate bioactive glass; Human adipose-derived stem cells Polymer/bioactive glass composite

International Standard Serial Number (ISSN)


Document Type

Article - Journal

Document Version

Final Version

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Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License

Publication Date

01 Jul 2019