The purpose of this project was to investigate the angiogenic mechanism of bioactive borate glass for soft tissue repair in a 'hairless' SKH1 mouse model. Subcutaneous microvascular responses to bioactive glass microfibers (45S5, 13-93B3, and 13-93B3Cu) and bioactive glass beads (13-93, 13-93B3, and 13-93B3Cu) were assessed via: noninvasive imaging of skin microvasculature; histomorphometry of microvascular densities; and quantitative PCR measurements of mRNA expression of VEGF and FGF-2 cytokines. Live imaging via dorsal skin windows showed the formation at two weeks of a halo-like structure infused with microvessels surrounding implanted borate-based 13-93B3 and 13-93B3Cu glass beads, a response not observed with silicate-based 13-93 glass beads. Quantitative histomorphometry of tissues implanted with plugs of 45S5, 13-93B3, and 13-93B3Cu glass microfibers revealed microvascular densities that were 1.6-, 2.3-, and 2.7-times higher, respectively, than the sham control values whereas 13-93, 13-93B3, and 13-93B3Cu glass beads caused the microvascular density to increase 1.3-, 1.6-, and 2.5-fold, respectively, relative to sham controls. Quantitative PCR measurements indicate a marginally significant increased expression of VEGF mRNA in tissues with 13-93B3Cu glass beads, an outcome that supported the hypothesis that copper-doped borate glass could promote VEGF expression followed by angiogenesis for enhanced wound healing.


Biological Sciences

Second Department

Materials Science and Engineering


This investigation was supported by funds from the Center for Biomedical Sciences and Engineering at Missouri University of Science and Technology. The bioactive glass materials used in this study were donated by the MO-Sci Corp in Rolla, MO.

Keywords and Phrases

Angiogenesis; Bioactive glass beads; Bioactive glass microfibers; Copper-doped bioactive borate glass; In vivo; Soft tissue

International Standard Serial Number (ISSN)


Document Type

Article - Journal

Document Version

Final Version

File Type





© 2015 Walter de Gruyter GmbH, All rights reserved.

Creative Commons Licensing

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.

Publication Date

01 Jan 2015