Zinc Oxide Nanoparticles Induce Oxidative Stress and Alter Calcium Homeostasis in Human Bronchial Epithelial Cells (BEAS-2B)
Abstract
The influence of 20 nm zinc oxide particles on intracellular calcium levels and gene expression was studied in human bronchial epithelial cells (BEAS-2B). There was a steep decline in cell numbers at concentration between 6-10 μg/mL. Intracellular ROS levels and LDH concentrations in the cell culture media were increased in dose-dependent manners. Exposure to ZnO increased intracellular calcium levels. Treatment with an antioxidant N-acetylcysteine (NAC) prevented cell loss indicating oxidative stress mediated cytotoxicity. Ca2+ concentrations were elevated by four fold compared to control cells and the elevation was partially attenuated when treated with NAC. Exposure to a sublethal dose of ZnO (5 μg/mL) increased the expression of BNIP, PRDX3, PRNP, and TXRND1 genes which were involved in apoptosis and OS responses. Thus, exposure of BEAS-2B cells to 20 nm ZnO results in 1) a dose- and time-dependent cytotoxicity reflected in cell viability reduction, elevated oxidative stress, and cell membrane damage, 2) a dose-dependent elevation of intracellular Ca2+ levels, and 3) several genes induced in apoptosis and OS responses.
Recommended Citation
C. Huang et al., "Zinc Oxide Nanoparticles Induce Oxidative Stress and Alter Calcium Homeostasis in Human Bronchial Epithelial Cells (BEAS-2B)," Free Radical Biology and Medicine, vol. 45, no. Supplement, Elsevier, Nov 2008.
The definitive version is available at https://doi.org/10.1016/j.freeradbiomed.2008.10.007
Meeting Name
15th Annual Meeting of the Society for Free Radical Biology and Medicine (2008: Nov. 19-23, Indianapolis, IN)
Department(s)
Biological Sciences
International Standard Serial Number (ISSN)
0891-5849
Document Type
Article - Conference proceedings
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2008 Elsevier, All rights reserved.
Publication Date
01 Nov 2008