Cellular Internalization of Quantum Dots

Abstract

Cell-penetrating peptides (CPPs) can facilitate uptake of quantum dots (QDs) for a variety of basic and applied sciences. Here we describe a method that utilizes simple noncovalent interactions to complex QDs and CPPs. We further describe methods to study uptake mechanisms of the QD/CPP complex. The inhibitor study coupled with the RNA interference (RNAi) technique provides a comprehensive approach to elucidate cellular entry of the QD/CPP complex.

Department(s)

Biological Sciences

Keywords and Phrases

Acrylamide; Caveolin 1; Chlorpromazine; Clathrin; Dansylcadaverine; Small Interfering RNA; Confocal Microscopy; Cytotoxicity; Endocytosis; Fluorescence Imaging; High Performance Liquid Chromatography; Image Quality; Internalization; Pinocytosis; Protein Binding; Receptor Down Regulation; Reversed Phase Liquid Chromatography; RNA Interference; Signal Transduction; Temperature; Western Blotting; Base Sequence; Quantum Dots; RNA, Small Interfering; Caveolin; Cell-Penetrating Peptides; Inhibitors; Lipid Raft; Macropinocytosis; siRNA

International Standard Book Number (ISBN)

978-1-62703-335-0; 978-1-62703-336-7

International Standard Serial Number (ISSN)

1064-3745

Electronic OCLC #

837693821

Print OCLC #

821217441

Document Type

Book - Chapter

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2013 Humana Press/Springer Verlag, All rights reserved.

Publication Date

01 Jan 2013

PubMed ID

23546675

Link to Full Text

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