A major limitation of using synthetic scaffolds in tissue engineering applications is insufficient angiogenesis in scaffold interior. Bioactive borate glasses have been shown to promote angiogenesis. There is a need to investigate the biofabrication of polymer composites by incorporating borate glass to increase the angiogenic capacity of the fabri-cated scaffolds. In this study, we investigated the bioprinting of human adipose stem cells (ASCs) with a polycaprolac-tone (PCL)/bioactive borate glass composite. Borate glass at the concentration of 10 to 50 weight %, was added to a mixture of PCL and organic solvent to make an extrudable paste. ASCs suspended in Matrigel were ejected as droplets using a second syringe. Scaffolds measuring 10 x 10 x 1 mm3 in overall dimensions with pore sizes ranging from 100 - 300 µm were fabricated. Degradation of the scaffolds in cell culture medium showed a controlled release of bioactive glass for up to two weeks. The viability of ASCs printed on the scaffold was investigated during the same time period. This 3D bioprinting method shows a high potential to create a bioactive, highly angiogenic three-dimensional environ-ment required for complex and dynamic interactions that govern the cell's behavior in vivo .
C. Murphy et al., "3D Bioprinting of Stem Cells and Polymer/Bioactive Glass Composite Scaffolds for Bone Tissue Engineering," International Journal of Bioprinting, vol. 3, no. 1, pp. 53 - 63, Whioce Publishing Pte. Ltd., Jan 2017.
The definitive version is available at https://doi.org/10.18063/IJB.2017.01.005
Materials Science and Engineering
Mechanical and Aerospace Engineering
Intelligent Systems Center
Keywords and Phrases
Bioactive glass; Biofabrication; Bioprinting; Human adipose-derived stem cell; MSCs; Polycaprolactone; Scaffold; Tissue engineering
International Standard Serial Number (ISSN)
Article - Journal
© 2017 Caroline Murphy, Krishna Kolan, Wenbin Li, Julie Semon, Delbert Day, Ming Leu, All rights reserved.
Creative Commons Licensing
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License
01 Jan 2017