Masters Theses

Shih-ying Sun

Abstract

"A porcine thyroid enzyme was extracted from thyroid tissue by homogenization and differential centrifugation. The enzyme was capable of metabolizing both 3-iodotyrosine and 3,5-diiodotyrosine. All of the thyroid subcellular particles produced a fluorescent compound when incubated with these substrates. When the 1,000 x g sediment was supplemented with the 48,000 x g supernatant a second compound was formed. The compound was UV absorbing and has been identified as either 3-iodo-4-hydroxybenzaldehyde or 3,5-diiodo-4-hydroxybenzaldehyde depending upon which substrate was used. The 1,000 x g sediment was solubilized with 1% Conoco 1012-6 detergent. The activating factor in the 48,000 x g supernatant was shown to be a macromolecule, probably a protein, by dialysis and ammonium sulfate precipitation. The enzymes in both fractions are required to form 3-iodo-4-hydroxybenzaldehyde. Conversion of the reaction products to their dinitrophenylhydrazones followed by paper chromatography indicated that the benzaldehydes were formed by decomposition of the corresponding p-hydroxyphenylpyruvic acid. This rules out the enzymatic formation of the benzaldehydes. This study lends support to model system studies which have suggested that 3,5 diiodo-4- hydroxyphenylpyruvic acid should be an intermediate in the formation of thyroxine"--Abstract, Page ii.

Advisor(s)

Nicholson, Larry M.

Committee Member(s)

Siehr, Donald J.
Wixson, Bobby G.

Department(s)

Chemistry

Degree Name

M.S. in Chemistry

Sponsor(s)

University of Missouri--Rolla. Department of Chemistry

Publisher

University of Missouri--Rolla

Publication Date

1970

Pagination

ix, 74 Pages

Note about bibliography

Includes bibliographical references (pages 45-48).

Rights

© 1970 Shih-ying Sun, All rights reserved.

Document Type

Thesis - Open Access

File Type

text

Language

English

Subject Headings

Tyrosine in the body
Thyroid gland -- Research

Thesis Number

T 2328

Print OCLC #

6013565

Electronic OCLC #

844776233

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