Masters Theses

Abstract

"The aim of this study was to investigate the effect of lipid composition on the physical properties of liposomes. Eight different types of liposomes were prepared for this study. Liposomes consisting of phosphatidylcholine (PC) and cholesterol were prepared in three different compositions 80/20, 50/50 and 20/80 respectively. Similarly liposomes consisting of PC and negatively charged phosphatidylserine (PS) were also prepared in three different compositions 80/20, 50/50 and 20/80 respectively. Two liposomes with the compositions 2:2:1:1 (cholesterol:PC:phosphatidylglycerol (PG): phosphatidylethanolamine (PE) ) which resembles the myelin membrane and 10:5:7.5:16 (PC:PE:PS:cholesterol) which mimics rat's neural membranes were also prepared. The liposomes were prepared by freeze-drying method. The experiments were carried out at three pHs 4, 7 and 9 and at three different temperatures 15⁰C, 25⁰C and 45⁰C. The ionic strength of the buffer used was 40mM. The size and polydispersity indices were measured by dynamic light scattering. The size of the liposomes obtained was small and reasonably monodispersed. Static light scattering experiments were performed using a Brookhaven temperature controlled goniometer equipped with a 2W argon ion laser. The data was analyzed to obtain second virial coefficients. The second virial coefficients obtained were positive for all liposomes at all pHs and all temperatures which indicates that the interactions are repulsive under these conditions. Zeta potentials of liposomes were determined using zetasizer nano ZS90. The zeta potentials obtained were all highly negative at above pHs and temperatures indicating that the liposomes were stable. The structure of the liposomes was analyzed using static light scattering by using Guinier and Kratky plots"--Abstract, page iii.

Advisor(s)

Forciniti, Daniel

Committee Member(s)

Al-Dahhan, Muthanna H.
Westenberg, David J.

Department(s)

Chemical and Biochemical Engineering

Degree Name

M.S. in Chemical Engineering

Publisher

Missouri University of Science and Technology

Publication Date

Spring 2012

Pagination

vii, 52 pages

Note about bibliography

Includes bibliographical references (pages 48-50).

Rights

© 2012 Rajiv Kumar Yandrapati, All rights reserved.

Document Type

Thesis - Open Access

File Type

text

Language

English

Subject Headings

Light -- Scattering
Liposomes
Virial coefficients
Zeta potential

Thesis Number

T 9992

Print OCLC #

815660194

Electronic OCLC #

773944550

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