Masters Theses

Abstract

“A membrane protein, cytochrome c reductase, was crystallized and examined using transmission electron microscopy. Two-dimensional crystals were prepared by two methods. In Method 1, the protein was solubilized in 50 mM Tris-Acetate (pH 6.0)/0.5 mM EDTA70.05% (w/v) Triton X-100 and reconstituted into a mixture of phosphatidylcholine and phosphatidylserine (4:1), dissolved in the same buffer. The Triton X-100 was removed from the mixture by dialysis in the presence of Bio-beads (SM-2) at approximately 25-30 °C. Method 2 was the same as Method 1, except that the vesicles were fractionated before reconstitution, and the pH and temperature for crystallization were 7.0 and 4 °C respectively.

After dialysis, the two samples were prepared for transmission electron microscopy by negatively staining the specimens on copper (400 mesh) grids. Electron micrographs of the proteoliposomes and single layered crystal sheets were taken along with their diffraction patterns. The proteoliposomes contained amorphous deposits of protein. The single layered sheets were highly ordered with protein. The unit cell for Method 1 could not be solved due to aggregation and overlapping of the crystal sheets. For Method 2, the unit cell is hexagonal, with p3 symmetry”--Abstract, page iii.

Advisor(s)

Forciniti, Daniel

Committee Member(s)

Reed, X. B., Jr.
Numbere, Daopu Thompson, 1951-

Department(s)

Chemical and Biochemical Engineering

Degree Name

M.S. in Chemical Engineering

Publisher

University of Missouri--Rolla

Publication Date

Fall 2000

Pagination

vii, 38 pages

Note about bibliography

Includes bibliographical references (pages 35-37).

Rights

© 2000 Sara Mia Pates, All rights reserved.

Document Type

Thesis - Open Access

File Type

text

Language

English

Thesis Number

T 7844

Print OCLC #

45902541

Download

Share

 
COinS