Keywords and Phrases
"Phenylalanine ammonia-lyase (PAL) (EC 4. 3. 1. 5) activity was greatly increased in Schizophyllum commune 4-40 by growing the organism in a medium containing L-Phenylalanine. S. commune 4-40 PAL was purified 19.2 fold by a procedure utilizing Sephadex G-50 chromatography, ammonium sulfate fractionation and chromatographic separation on Sephadex G-200. The enzyme has a native molecular weight of 247,000. The Km value for L-phenylalanine is 0.232 mM, the Vmax is 0.415 mM/minute and the kcat is 0.0164/second for the purified lyase.
Two attempts were made to synthesize a cDNA library from mRNA isolated from S. commune 4-40. Soybean PAL DNA and Rhodotorula rubra PAL DNAs were used as probes for screening for the PAL gene in the cDNA libraries and a genomic library prepared by Specht and Ulrich. The inability to cut the DNA isolated from reconstituted phage supposedly containing DNA from the cDNA library into more than 2 fragments with a mixture of two restriction endonucleases plus the high incidence of positive plaques in the screening tests, suggest that a true library had not been synthesized"--Abstract, page iii.
Siehr, Donald J.
Hanna, Samir B.
Frank, Ronald L.
Ph. D. in Chemistry
University of Missouri--Rolla
x, 111 leaves
© 1993 Jiamin Zeng, All rights reserved.
Dissertation - Restricted Access
Library of Congress Subject Headings
DNA -- Analysis
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Electronic access to the full-text of this document is restricted to Missouri S&T users. Otherwise, request this publication directly from Missouri S&T Library or contact your local library.http://laurel.lso.missouri.edu/record=b2668576~S5
Zeng, Jiamin, "The isolation and characterization of phenylalanine ammonia lyase from Schizophyllum commune and an attempt to isolate the gene" (1993). Doctoral Dissertations. 1019.
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