A Simple and Fast Method to Determine and Quantify Urinary Creatinine
A simple and fast method for the determination of urinary creatinine by high-performance capillary electrophoresis (HPCE) has been developed. The urine samples were diluted 50 fold before they were injected onto an HPCE column for analysis. with this technique, the urinary creatinine level can be determined within 4 min. The interference from urea in urine has been totally eliminated. The reproducibility and recovery of the technique have been studied and it was proven that the technique is satisfactory for quantitative determination of urine creatinine. The results obtained from this technique have been compared with results obtained by high-performance liquid chromatography, and the results from both techniques agreed quite well (correlation coefficient = 0.993). The linear range of the technique was over two orders of magnitude (0.1-11 mg/dl) which covers the creatinine concentration in human urine after 50 fold dilution. The detection limit for creatinine is 0.04 mg/dl with a signal-to-noise ratio of 3.
H. Shi et al., "A Simple and Fast Method to Determine and Quantify Urinary Creatinine," Analytica Chimica Acta, vol. 312, no. 1, pp. 79-83, Elsevier Masson, Aug 1995.
The definitive version is available at https://doi.org/10.1016/0003-2670(95)00208-H
Keywords and Phrases
Creatinine Urine Level; Human Tissue; Methodology; Priority Journal; Quantitative Assay; Zone Electrophoresis; Electrophoresis; High-performance Liquid Chromatography; Urine
International Standard Serial Number (ISSN)
Article - Journal
© 1995 Elsevier Masson, All rights reserved.