Abstract
The redox-neutral reaction catalyzed by 2-haloacrylate hydratase (2-HAH) leads to the conversion of 2-chloroacrylate to pyruvate. Previous mechanistic studies demonstrated the formation of a flavin-iminium ion as an important intermediate in the 2-HAH catalytic cycle. Time-resolved flavin absorbance studies were performed in this study, and the data showed that the enzyme is capable of stabilizing both anionic and neutral flavin semiquinone species. The presence of a radical scavenger decreases the activity in a concentration-dependent manner. These data are consistent with the flavin iminium intermediate occurring by radical recombination.
Recommended Citation
Y. Dai et al., "Evidence for the Formation of a Radical-Mediated Flavin-N5 Covalent Intermediate," ChemBioChem, vol. 19, no. 15, pp. 1609 - 1612, Wiley; Wiley-VCH Verlag, Aug 2018.
The definitive version is available at https://doi.org/10.1002/cbic.201800123
Department(s)
Chemistry
Publication Status
Full Access
Keywords and Phrases
covalent adducts; flavin; non-redox reaction; radicals; semiquinones
International Standard Serial Number (ISSN)
1439-7633; 1439-4227
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2024 Wiley; Wiley-VCH Verlag, All rights reserved.
Publication Date
06 Aug 2018
PubMed ID
29776001
Comments
National Science Foundation, Grant 1506206