Abstract
The high-performance liquid chromatographic enantioresolution of β- substituted tryptophan analogues by direct and indirect methods is reported. The direct separation was carried out on chiral crown ether-bonded, on the macrocyclic antibiotic teicoplanin-bonded or on different derivatized β- cyclodextrin-bonded phases. The indirect resolution was achieved by applying pre-column derivatization with the chiral reagents 1-fluoro-2,4- dinitrophenyl-5-L-alanine amide (FDAA) and 2,3,4,6-tetra-O-acetyl-β-D- glucopyranosyl isothiocyanate (GITC). In the separation of all four isomers of the analytes, the indirect methods seemed to be more efficient than the direct methods. In most cases, the FDAA derivatives of the stereoisomers could be separated with better selectivity in methanol-containing mobile phases.
Recommended Citation
G. Török et al., "Enantiomeric High-performance Liquid Chromatographic Separation of Β- Substituted Tryptophan Analogues," Chromatographia, vol. 51, no. 1, Springer, Jan 2000.
The definitive version is available at https://doi.org/10.1007/bf02492800
Department(s)
Chemistry
Keywords and Phrases
Column liquid chromatography; Enantiomeric separation; β-Substituted tryptophan analogues
International Standard Serial Number (ISSN)
0009-5893
Document Type
Article - Conference proceedings
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2024 Springer, All rights reserved.
Publication Date
01 Jan 2000
