Abstract
Recent advances in the technique of capillary electrophoresis have demonstrated fast, highly efficient separation of mixtures of intact microbes. This paper describes the application of this technique for the separation of microbial aggregates of Micrococcus luteus, Saccharomyces cerevisiae, or Alcaligenes faecalis. The aggregates of these microbes were resolved into several highly efficient peaks with analysis times under 10 min and efficiencies approaching 1 000 000 plates m-1 in some cases. A reproducible relationship was found between the electrophoretic mobility and the aggregation number or size of the cluster under a given set of experimental conditions. Often, cellular aggregation was reversible with brief immersion in an ultrasound bath. This reversibility was confirmed by visual microscopy and electrophoretic data. Copyright (C) 2000 Federation of European Microbiological Societies.
Recommended Citation
J. M. Schneiderheinze et al., "High Efficiency Separation of Microbial Aggregates using Capillary Electrophoresis," FEMS Microbiology Letters, vol. 189, no. 1, pp. 39 - 44, Oxford University Press; Federation of European Microbiological Societies, Aug 2000.
The definitive version is available at https://doi.org/10.1016/S0378-1097(00)00249-4
Department(s)
Chemistry
Second Department
Biological Sciences
Third Department
Chemical and Biochemical Engineering
Publication Status
Free Access
Keywords and Phrases
Bacterium; Capillary electrophoresis characterization; Polymer; Separation; Yeast
International Standard Serial Number (ISSN)
0378-1097
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2024 Oxford University Press; Federation of European Microbiological Societies, All rights reserved.
Publication Date
01 Aug 2000
PubMed ID
10913863
