Abstract
The use of high-performance capillary electrokinetic techniques for the separation, identification, and quantitation of intact microbes represents a new frontier for separation science. In this work, it is demonstrated that pathogens most responsible for urinary tract infections can be distinguished from one another after direct injection of untreated urine. High efficiencies (often exceeding 1 000 000 plates/m) and short analysis times (< 10 min) are characteristics of this approach. The concentration of the urine matrix appears to be able to cause a small, but definite, change in the electroosmotic flow velocity. This high-efficiency separation-based approach could prove to be invaluable for the diagnosis and tracking of certain diseases. It also could form the basis for a variety of rapid microbial assays.
Recommended Citation
D. W. Armstrong and J. M. Schneiderheinze, "Rapid Identification of the Bacterial Pathogens Responsible for Urinary Tract Infections using Direct Injection CE," Analytical Chemistry, vol. 72, no. 18, pp. 4474 - 4476, American Chemical Society, Sep 2000.
The definitive version is available at https://doi.org/10.1021/ac000302j
Department(s)
Chemistry
International Standard Serial Number (ISSN)
0003-2700
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2024 American Chemical Society, All rights reserved.
Publication Date
15 Sep 2000
PubMed ID
11008786
