High-performance Liquid Chromatographic Separation of Enantiomers of Synthetic Amino Acids on a Ristocetin a Chiral Stationary Phase

Author

Antal Péter
Gabriella Török
Daniel W. Armstrong, Missouri University of Science and Technology
Géza Tóth
Dirk Tourwé

Abstract

A macrocyclic glycopeptide, ristocetin A, was used as chiral stationary phase for the high-performance liquid chromatographic separation of enantiomers of 28 unnatural amino acids, such as analogues of phenylalanine, tyrosine and tryptophan, and analogues containing 1,2,3,4-tetrahydroisoquinoline, tetraline or 1,2,3,4-tetrahydro-2-carboline skeletons. Excellent resolutions were achieved for most of the investigated compounds by using reversed-phase or a new polar-organic mobile phase system. The conditions of separation were optimized by variation of the mobile phase composition, temperature and flowrate. Copyright (C) 2000 Elsevier Science B.V.

Recommended Citation

A. Péter et al., "High-performance Liquid Chromatographic Separation of Enantiomers of Synthetic Amino Acids on a Ristocetin a Chiral Stationary Phase," Journal of Chromatography A, vol. 904, no. 1, pp. 1 - 15, Elsevier, Dec 2000.

The definitive version is available at https://doi.org/10.1016/S0021-9673(00)00917-1

Department(s)

Chemistry

Comments

National Institutes of Health, Grant ROI GM 53825-01

Keywords and Phrases

Amino acids; Chiral stationary phases, LC; Enantiomer separation; Ristocetin A

International Standard Serial Number (ISSN)

0021-9673

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2024 Elsevier, All rights reserved.

Publication Date

22 Dec 2000

PubMed ID

11209895