Abstract
A glycopeptide antibiotic, teicoplanin, was used as chiral stationary phase for the high-performance liquid chromatographic (HPLC) separation of enantiomers of more than 30 unnatural amino acids, such as phenylalanine and tyrosine analogues and analogues containing 1,2,3,4-tetrahydroisoquinoline, tetraline, 1,2,3,4-tetrahydro-2-carboline, cyclopentane, cyclohexane, cyclohexene, bicyclo[2.2.1]heptane or heptene skeletons. Excellent resolutions were achieved for most of the investigated compounds by using a hydro-organic mobile-phase system. The effects of organic modifier content, temperature and flow-rate on the resolution were investigated and the conditions of separation were optimized.
Recommended Citation
A. Péter et al., "High-Performance Liquid Chromatographic Separation of Enantiomers of Unusual Amino Acids on a Teicoplanin Chiral Stationary Phase," Journal of Chromatography A, vol. 793, no. 2, pp. 283 - 296, Elsevier, Jan 1998.
The definitive version is available at https://doi.org/10.1016/S0021-9673(97)00938-2
Department(s)
Chemistry
Keywords and Phrases
Amino acids; Chiral stationary phases, LC; Enantiomer separation; Teicoplanin
International Standard Serial Number (ISSN)
0021-9673
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2024 Elsevier, All rights reserved.
Publication Date
16 Jan 1998
PubMed ID
9474786
Comments
National Institutes of Health, Grant 252/96