Abstract
High-performance liquid chromatographic and gas chromatographic methods were developed for the separation of unusual secondary aromatic amino acids. Amino acids containing 1,2,3,4-tetrahydroisoquinoline, 1,2,3,4-tetrahydronorharmane-1-carboxylic acid and 1,2,3,4-tetrahydro-3-carboxy-2-carboline moieties were synthetized in racemic or chiral forms. The high-performance liquid chromatography was carried out either on a teicoplanin-containing chiral stationary phase or on an achiral C18 column. In the latter case the diastereomers of the amino acids formed by precolumn derivatization with the chiral reagents 2,3,3,6-tetra-O-acetyl-β-D-glucopyranosyl isothiocyanate or 1-fluoro-2,4-dinitrophenyl-5-L-alanine amide were separated. The gas chromatographic analyses were based on separation on a Chirasil-L-Val column.
Recommended Citation
A. Péter et al., "Enantiomeric Separation of Unusual Secondary Aromatic Amino Acids," Chromatographia, vol. 48, no. 1 thru 2, pp. 53 - 58, Springer, Jul 1998.
The definitive version is available at https://doi.org/10.1007/BF02467516
Department(s)
Chemistry
Keywords and Phrases
Chiral derivatization; Column liquid chromatography; Enantiomeric separation; Gas chromatography; Unusual secondary aromatic amino acids
International Standard Serial Number (ISSN)
0009-5893
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2024 Springer, All rights reserved.
Publication Date
29 Jul 1998
Comments
National Institutes of Health, Grant B-3/96