Title

Specific Intracellular Uptake of Herceptin-Conjugated CdSe/ZnS Quantum Dots into Breast Cancer Cells

Abstract

Herceptin, a typical monoclonal antibody, was immobilized on the surface of CdSe/ZnS core-shell quantum dots (QDs) to enhance their specific interactions with breast cancer cells (SK-BR3). the mean size of the core-shell quantum dots (28 nm), as determined by dynamic light scattering, increased to 86 nm after herceptin immobilization. the in vitro cell culture experiment showed that the keratin forming cancer cells (KB) proliferated well in the presence of herceptin-conjugated QDs (QD-Her, 5 nmol/mL), whereas most of the breast cancer cells (SK-BR3) had died. to clarify the mechanism of cell death, the interaction of SK-BR3 cells with QD-Her was examined by confocal laser scanning microscopy. as a result, the QD-Her bound specifically to the membrane of SK-BR3, which became almost saturated after 6 hours incubation. This suggests that the growth signal of breast cancer cells is inhibited completely by the specific binding of herceptin to the Her-2 receptor of SK-BR3 membrane, resulting in cell death.

Department(s)

Chemistry

Keywords and Phrases

cadmium selenide; cadmium selenide zinc sulfide quantum dot; keratin; quantum dot; trastuzumab; unclassified drug; zinc sulfide; cadmium derivative; cadmium selenide; monoclonal antibody; quantum dot; selenium derivative; sulfide; trastuzumab; zinc derivative; zinc sulfide, article; breast cancer; cancer cell; cancer cell culture; cancer mortality; cell interaction; cell membrane; cell proliferation; confocal laser microscopy; controlled study; drug binding; drug potentiation; dynamic light scattering; in vitro study; infrared spectroscopy; light scattering; particle size; ultraviolet spectroscopy; zeta potential; breast tumor; cell survival; chemistry; drug effect; female; human; metabolism; tumor cell line, Antibodies, Monoclonal, Humanized; Breast Neoplasms; Cadmium Compounds; Cell Line, Tumor; Cell Survival; Female; Humans; Particle Size; Quantum Dots; Selenium Compounds; Spectroscopy, Fourier Transform Infrared; Sulfides; Zinc Compounds

International Standard Serial Number (ISSN)

2314-6133

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2014 Hindawi Publishing Corporation, All rights reserved.


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