Abstract
A theoretical formulation is presented that can be used to describe the dynamic behavior of frontal chromatography of proteins in columns packed with adsorbent particles in which restricted pore diffusion of the adsorbate molecules occurs. The results of this work clearly indicate that the time for breakthrough and the effective utilization of the adsorptive capacity of the chromatographic particles increase as (a) the size of the adsorbate and/or ligand (active site) molecule decreases, (b) the pore connectivity, n(T), of the porous network of the adsorbent particles increases, and (c) the column length, L, increases. Copyright (C) 1998 Elsevier Science B.V.
Recommended Citation
A. I. Liapis et al., "Frontal Chromatography of Proteins: The Effect on Column Performance of the Restricted Diffusion of Molecules in Porous Chromatographic Adsorbents," Journal of Chromatography A, vol. 828, no. 1 thru 2, pp. 345 - 356, Elsevier, Dec 1998.
The definitive version is available at https://doi.org/10.1016/S0021-9673(98)00642-6
Department(s)
Chemical and Biochemical Engineering
Keywords and Phrases
Adsorbents; Frontal chromatography; Proteins; Restricted pore diffusion
International Standard Serial Number (ISSN)
0021-9673
Document Type
Article - Conference proceedings
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2024 Elsevier, All rights reserved.
Publication Date
18 Dec 1998
