In Vitro Cellular Response to Hydroxyapatite Scaffolds with Oriented Pore Architectures
Abstract
The objective of the present work was to evaluate the in vitro cellular response to hydroxyapatite (HA) scaffolds with oriented pore architectures. Hydroxyapatite scaffolds with approximately the same porosity (65-70%) but two different oriented microstructures, described as 'columnar' (pore diameter = 90-110 μm) and 'lamellar' (pore width = 20-30 μm), were prepared by unidirectional freezing of suspensions. The response of murine MLO-A5 cells, an osteogenic cell line, to these scaffolds was evaluated using assays of MTT hydrolysis, alkaline phosphatase (ALP) activity, and alizarin red staining. While the cellular response to both groups of scaffolds was better than control wells, the columnar scaffolds with the larger pore width provided the most favorable substrate for cell proliferation and function. These results indicate that HA scaffolds with the columnar microstructure could be used for bone repair applications in vivo.
Recommended Citation
Q. Fu et al., "In Vitro Cellular Response to Hydroxyapatite Scaffolds with Oriented Pore Architectures," Materials Science and Engineering: C., vol. 29, no. 7, pp. 2147 - 2153, Elsevier, Aug 2009.
The definitive version is available at https://doi.org/10.1016/j.msec.2009.04.016
Department(s)
Biological Sciences
Second Department
Materials Science and Engineering
Keywords and Phrases
Cell culture; Hydroxyapatite
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2009 Elsevier, All rights reserved.
Publication Date
01 Aug 2009