Title

The F₄₂₀H₂-dehydrogenase from Methanolobus Tindarius: Cloning of the Ffd Operon and Expression of the Genes in Escherichia Coli

Abstract

The membrane-bound F420H2-dehydrogenase from the methylotrophic methanogen Methanolobus tindarius oxidizes reduced coenzyme F420 and feeds the electrons into an energy-conserving electron transport chain. Based on the N-terminal amino acid sequence of the 40-kDa subunit of F420H2-dehydrogenase the corresponding gene ffdB was detected in chromosomal DNA of M. tindarius. Sequence analysis, primer extension, and RT-PCR experiments indicated that ffdB is part of an operon harboring three additional open reading frames (ffdA, ffdC, ffdD). The corresponding mRNA transcript and transcription start sites were determined. All four genes could be heterologously expressed in Escherichia coli.

Department(s)

Biological Sciences

Sponsor(s)

German Research Association
Fonds der Chemischen Industrie

Comments

The financial support by the "Deutsche Forschungsgemeinschaft" and "Fonds der ChemischenIndustrie" is gratefully acknowledged.

Keywords and Phrases

Electron Transport; F420H2-dehydrogenase; ffdB Gene; Heterologous Expression; Methanolobus tindarius

International Standard Serial Number (ISSN)

0378-1097; 1574-6968

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 1999 John Wiley & Sons, All rights reserved.

PubMed ID

9933933

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