Title

Advancement of the Biosynthetic Plant Registry

Presenter Information

Mikayla Tessmer

Department

Chemical and Biochemical Engineering

Major

Chemical Engineering (Biochemical Engineering Emphasis)

Research Advisor

Westenberg, David J.

Advisor's Department

Biological Sciences

Funding Source

Dr. David Westerberg and MS&T IGEM Design Team

Abstract

The 2018 International Genetically Engineered Machines (iGEM) Jamboree conference proved to show flaws in the international plant registry. Although the iGEM registry has over twenty thousand documented parts, only a small percentage make up the plant registry section. This research focused on adding known promoters to the registry to improve future use and documentation. The AT5G54000 promoter is a seed-specific promoter, therefore any gene put after the promoter will affect only the seed. The GmPRP2 promoter is a root-specific promoter. To test the promoters activity, it was combined with the GUS reporter which produces a blue coloration in the specific tissue when given X-gluc. Each promoter's effects can be properly documented. By putting the combination into the specific iGEM backbone, it can be added to the iGEM plant registry to make future projects less time consuming.

Biography

Mikayla is a junior in Chemical Engineering that has done undergraduate research in the past with Dr. Westenberg at MS&T and the National Science Foundation at the University of Houston. She is involved on campus as the secretary of Omega Chi Epsilon, the chemical engineering honor society. Additionally, she participates with the iGEM design team and Chi Omega greek sorority.This upcoming summer (2019) she will be interning with Bayer to do research on plant pathogens.

Presentation Type

OURE Fellows Final Oral Presentation

Document Type

Presentation

Location

Missouri Room

Presentation Date

16 Apr 2019, 10:30 am - 11:00 am

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Apr 16th, 10:30 AM Apr 16th, 11:00 AM

Advancement of the Biosynthetic Plant Registry

Missouri Room

The 2018 International Genetically Engineered Machines (iGEM) Jamboree conference proved to show flaws in the international plant registry. Although the iGEM registry has over twenty thousand documented parts, only a small percentage make up the plant registry section. This research focused on adding known promoters to the registry to improve future use and documentation. The AT5G54000 promoter is a seed-specific promoter, therefore any gene put after the promoter will affect only the seed. The GmPRP2 promoter is a root-specific promoter. To test the promoters activity, it was combined with the GUS reporter which produces a blue coloration in the specific tissue when given X-gluc. Each promoter's effects can be properly documented. By putting the combination into the specific iGEM backbone, it can be added to the iGEM plant registry to make future projects less time consuming.