Title

PolyBall -- A New Adsorbent for the Removal Of Endotoxin

Presenter Information

Dibbya Barua

Department

Electrical and Computer Engineering

Major

Computer Engineering

Research Advisor

Barua, Sutapa

Advisor's Department

Chemical and Biochemical Engineering

Funding Source

Start-up, technology acceleration grant and S&T innovation

Abstract

In biotechnology industries, gram-negative bacteria are widely used for the production of therapeutic biomolecules including proteins, peptides, and nucleic acids. These biomolecules are recovered by cellular rupturing that leads to the release of a large quantity of bacterial cell-wall components containing endotoxins, also known as lipopolysaccharides (LPS). When the LPS contaminated products are administered to animals or humans even in small quantities (0.05–0.1 ng/ml), a systemic inflammatory reaction can occur, leading to multiple pathophysiological effects, such as septic shock, tissue injury, and lethality. Removing undesirable endotoxins from solutions is thus an important aim in the pharmaceutical industry and in clinical practice. Conventional treatments such as coagulation and membrane filtration are adequate for removing bacteria cells and debris but not effective for removing dissolved endotoxins to a significant extent. Therefore, it is highly desirable and also the focus of this project to develop a biodegradable and inexpensive means that can tackle both aspects of endotoxin removal.

Biography

Dibbya Barua is a Freshman Engineering student who would like to gain research experience in data analysis. His research interests include computational programming by virtual visualization of a specific research problem. Through this OURE project, he will develop software programs to understand the mechanisms of endotoxin binding with polymer nanoparticles. He is a recipient of several awards that include S&T's opening week car design, Regional Math Olympiads and 1st place in High School Science Fair.

Research Category

Engineering

Presentation Type

Poster Presentation

Document Type

Poster

Award

Engineering poster session, Second place

Location

Upper Atrium

Presentation Date

16 Apr 2019, 9:00 am - 3:00 pm

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Apr 16th, 9:00 AM Apr 16th, 3:00 PM

PolyBall -- A New Adsorbent for the Removal Of Endotoxin

Upper Atrium

In biotechnology industries, gram-negative bacteria are widely used for the production of therapeutic biomolecules including proteins, peptides, and nucleic acids. These biomolecules are recovered by cellular rupturing that leads to the release of a large quantity of bacterial cell-wall components containing endotoxins, also known as lipopolysaccharides (LPS). When the LPS contaminated products are administered to animals or humans even in small quantities (0.05–0.1 ng/ml), a systemic inflammatory reaction can occur, leading to multiple pathophysiological effects, such as septic shock, tissue injury, and lethality. Removing undesirable endotoxins from solutions is thus an important aim in the pharmaceutical industry and in clinical practice. Conventional treatments such as coagulation and membrane filtration are adequate for removing bacteria cells and debris but not effective for removing dissolved endotoxins to a significant extent. Therefore, it is highly desirable and also the focus of this project to develop a biodegradable and inexpensive means that can tackle both aspects of endotoxin removal.