Title

The Simultaneous Identification of Urinary Biomarkers for Early Detection of Traumatic Brain Injury

Presenter Information

Alexandre Cristea

Department

Chemistry

Major

Biochemistry

Research Advisor

Ma, Yinfa

Advisor's Department

Chemistry

Funding Source

National Institutes of Health (NIH)

Abstract

Over 2.5 million cases of traumatic brain injury (TBI) are reported each year in the United States and are involved in 30% of all injury-related deaths. TBI’s can occur in a variety of ways and are thus difficult to diagnose. Current methods either include extensive imaging using expensive equipment or simple qualitative assessments that analyze a patient’s verbal and motor skill. Due to the lack of an efficient method of TBI characterization, a metabolomics approach will be taken in order to design a biomarker profile for the complex injury. By utilizing high-performance liquid chromatography - tandem mass spectroscopy (HPLC - MS/MS) in addition to enzyme-linked immunosorbent assays (ELISA), a quantitative method will be designed to analyze the concentrations of 20 compounds found in urine. This method will then be used to analyze urine samples from 150 patients with severe TBIs, 150 patients with mild TBIs, and 150 matched controls. This data will then undergo statistical analysis in an attempt to link the relative concentrations of the 20 chemical panel with TBIs.

Biography

Alex is a junior biochemistry student who has been involved in Dr. Ma’s group since December of 2015. His research has primarily focused on bioanalytical chemistry and biomedical chemistry, specifically biomarker discovery, cell migration patterns, and cytotoxicity of nanoparticles to human cells. In addition to undergraduate research, he is also involved in the Missouri S&T Student Council and is the current treasurer.

Presentation Type

OURE Fellows Proposal Oral Applicant

Document Type

Presentation

Award

2016-2017 OURE Fellows recipient

Location

Turner Room

Presentation Date

17 Apr 2018, 1:20 pm - 1:40 pm

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Apr 17th, 1:20 PM Apr 17th, 1:40 PM

The Simultaneous Identification of Urinary Biomarkers for Early Detection of Traumatic Brain Injury

Turner Room

Over 2.5 million cases of traumatic brain injury (TBI) are reported each year in the United States and are involved in 30% of all injury-related deaths. TBI’s can occur in a variety of ways and are thus difficult to diagnose. Current methods either include extensive imaging using expensive equipment or simple qualitative assessments that analyze a patient’s verbal and motor skill. Due to the lack of an efficient method of TBI characterization, a metabolomics approach will be taken in order to design a biomarker profile for the complex injury. By utilizing high-performance liquid chromatography - tandem mass spectroscopy (HPLC - MS/MS) in addition to enzyme-linked immunosorbent assays (ELISA), a quantitative method will be designed to analyze the concentrations of 20 compounds found in urine. This method will then be used to analyze urine samples from 150 patients with severe TBIs, 150 patients with mild TBIs, and 150 matched controls. This data will then undergo statistical analysis in an attempt to link the relative concentrations of the 20 chemical panel with TBIs.