Title

Portable Biofiltration Kit

Presenter Information

Alexandria Lore

Department

Chemical and Biochemical Engineering

Major

Chemical Engineering

Research Advisor

Barua, Sutapa

Advisor's Department

Chemical and Biochemical Engineering

Funding Source

PI’s Start Up, Missouri S&T’s Innovation Award (Miner Tank) 2018

Abstract

Endotoxin removal is an important but understudied process of purification. Endotoxins are released from gram-negative and gram-positive bacteria. The most common sources of infections are caused by endotoxins from gram-negative bacteria and can cause sepsis and toxic shock when a large amount of these infect the body. Endotoxins prove difficult to remove from solutions due to their size and physical and chemical properties. Current methods for endotoxin removal are often inefficient, expensive, or require a laboratory setting and a power source to operate. The proposed biofiltration kit would provide a portable membrane that could be used with gravity to filter endotoxins from water. Microparticles are present in the membrane which bind to endotoxins using electrostatic and hydrophobic properties. Water flux was taken with only the driving force of gravity over several hours, and endotoxin removal was measured using a fluorescent assay to determine overall effectiveness of the microparticle membrane.

Biography

Alexandria Lore is a Senior in Chemical Engineering at Missouri S&T where she works on Biopharmaceutical research with Dr. Sutapa Barua. She serves as a member of the executive board of the St. Pat’s Board as secretary and is an alumna of Lambda Sigma Pi, service organization. She also graduated with a B.S. in Biological Sciences in 2013 from Missouri S&T.

Research Category

Engineering

Presentation Type

Oral Presentation

Document Type

Presentation

Location

Carver Room

Presentation Date

17 Apr 2018, 10:30 am - 11:00 am

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Apr 17th, 10:30 AM Apr 17th, 11:00 AM

Portable Biofiltration Kit

Carver Room

Endotoxin removal is an important but understudied process of purification. Endotoxins are released from gram-negative and gram-positive bacteria. The most common sources of infections are caused by endotoxins from gram-negative bacteria and can cause sepsis and toxic shock when a large amount of these infect the body. Endotoxins prove difficult to remove from solutions due to their size and physical and chemical properties. Current methods for endotoxin removal are often inefficient, expensive, or require a laboratory setting and a power source to operate. The proposed biofiltration kit would provide a portable membrane that could be used with gravity to filter endotoxins from water. Microparticles are present in the membrane which bind to endotoxins using electrostatic and hydrophobic properties. Water flux was taken with only the driving force of gravity over several hours, and endotoxin removal was measured using a fluorescent assay to determine overall effectiveness of the microparticle membrane.