Title

MP3: Microparticle-laden Protein Particles for Wound Healing

Presenter Information

Elizabeth Lemieux

Department

Chemical and Biochemical Engineering

Major

Chemical Engineering

Research Advisor

Barua, Sutapa

Advisor's Department

Chemical and Biochemical Engineering

Funding Source

University of Missouri Research Board

Abstract

The objective of this research is to synthesize biodegradable microparticles (MP3s) to serve as a structural matrix for human cells, with the end goal of application for burn wound healing. The MP3s will be synthesized using poly(lactic-co-glycolic acid) (PLGA) polymer using the single emulsion solvent-evaporation technique while incorporating a proprietary flowfocusing device. Fibronectin will be immobilized on the surface of MP3s using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and Nhydroxysuccinimide (NHS) conjugation and quantified using the bicinchoninic acid (BCA) assay. The conjugated MP3s will be seeded with human umbilical vein endothelial cells (HUVECs) and assessed for cell binding ability. The viable cell attachment and proliferation will be qualitatively analyzed using fluorescent microscopy. It is expected that fibronectin immobilization on the surface of MP3s will increase cell attachment compared to unmodified particles or particles coated with other proteins. The future of this project will be the study of effects of MP3s for tissue regeneration.

Biography

Elizabeth Lemieux is a sophomore in Chemical Engineering at Missouri S&T, pursuing minors in Chemistry and Nuclear Engineering. She is a member of Chi Omega Fraternity, Society of Women Engineers, and is on the executive board for Engineers Without Borders. Elizabeth hopes to graduate in May 2020.

Research Category

Engineering

Presentation Type

Poster Presentation

Document Type

Poster

Location

Upper Atrium

Presentation Date

17 Apr 2018, 1:00 pm - 4:00 pm

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Apr 17th, 1:00 PM Apr 17th, 4:00 PM

MP3: Microparticle-laden Protein Particles for Wound Healing

Upper Atrium

The objective of this research is to synthesize biodegradable microparticles (MP3s) to serve as a structural matrix for human cells, with the end goal of application for burn wound healing. The MP3s will be synthesized using poly(lactic-co-glycolic acid) (PLGA) polymer using the single emulsion solvent-evaporation technique while incorporating a proprietary flowfocusing device. Fibronectin will be immobilized on the surface of MP3s using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and Nhydroxysuccinimide (NHS) conjugation and quantified using the bicinchoninic acid (BCA) assay. The conjugated MP3s will be seeded with human umbilical vein endothelial cells (HUVECs) and assessed for cell binding ability. The viable cell attachment and proliferation will be qualitatively analyzed using fluorescent microscopy. It is expected that fibronectin immobilization on the surface of MP3s will increase cell attachment compared to unmodified particles or particles coated with other proteins. The future of this project will be the study of effects of MP3s for tissue regeneration.