Title

Comparing Phenotypes of IQG1 Mutants

Presenter Information

Madison Mara

Department

Biological Sciences

Major

Biological Sciences

Research Advisor

Shannon, Katie

Advisor's Department

Biological Sciences

Funding Source

OURE

Abstract

During cytokinesis, many mutations can arise that affect formation of the actomyosin ring. Iqg1 is a protein that is required for assembly and contraction of the actomyosin ring in budding yeast. This project is designed to compare the phenotypes of three separate IQG1 mutant alleles to examine any problems that arise during cytokinesis. In this research, a mutant (2A) that has two serines mutated to alanine and a mutant (4A) that has three serines and one threonine mutated to alanine are compared in relation to the wild type strain. Investigation of another mutant (3T) has 3 serines mutated to theronines that is able to be phosphorylated but unable to be dephosphorylated, which is suspected to cause a lethal phenotype. The goal in doing this is to compare cytokinesis defects in the 2A mutant, 4A mutant, and 3T mutant and see if threonine has a unique function and determine the phenotype of permanent phosphorylation. To confirm mutant phenotype, morphological analysis will be performed via microscopy and immunofluorescence to indicate if actin ring formation has been disrupted.

Biography

Madison is a Senior at Missouri University of Science and Technology pursuing a degree in Biological Sciences with an emphasis in pre medicine. She is a member of Scrubs Pre-Med Club, Zeta Tau Alpha, Phi Sigma Biological Honor Society, and has been conducting research in Dr. Shannon’s cytokinesis lab for three years. She plans on pursuing a MD/Ph.D. once she graduates from Missouri S&T.

Research Category

Sciences

Presentation Type

Oral Presentation

Document Type

Presentation

Location

Carver Room

Start Date

4-11-2017 10:20 AM

End Date

4-11-2017 10:40 AM

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Apr 11th, 10:20 AM Apr 11th, 10:40 AM

Comparing Phenotypes of IQG1 Mutants

Carver Room

During cytokinesis, many mutations can arise that affect formation of the actomyosin ring. Iqg1 is a protein that is required for assembly and contraction of the actomyosin ring in budding yeast. This project is designed to compare the phenotypes of three separate IQG1 mutant alleles to examine any problems that arise during cytokinesis. In this research, a mutant (2A) that has two serines mutated to alanine and a mutant (4A) that has three serines and one threonine mutated to alanine are compared in relation to the wild type strain. Investigation of another mutant (3T) has 3 serines mutated to theronines that is able to be phosphorylated but unable to be dephosphorylated, which is suspected to cause a lethal phenotype. The goal in doing this is to compare cytokinesis defects in the 2A mutant, 4A mutant, and 3T mutant and see if threonine has a unique function and determine the phenotype of permanent phosphorylation. To confirm mutant phenotype, morphological analysis will be performed via microscopy and immunofluorescence to indicate if actin ring formation has been disrupted.