Title

Cytokinesis and the effect of mutations on protein-protein interactions

Presenter Information

Caitlin Siehr

Department

Biological Sciences

Major

Biological Sciences

Research Advisor

Shannon, Katie

Advisor's Department

Biological Sciences

Funding Source

OURE

Abstract

Iqg1 is a protein involved in cytokinesis in budding yeast. It is required for the assembly and contraction of the actin ring- which is responsible for dividing the two cells. In previous studies, Iqg1 has been shown to interact with the two formin proteins Bni1 and Bnr1. The overall objective of my research is to determine how mutations affecting Iqg1 phosphorylation alter protein-protein interactions. My research began with a preparation of yeast extracts from three different strains- a wild type, a mutant that prevents Iqg1 phosphorylation, and a mutant that mimics Iqg1 phosphorylation. I then used these extracts to perform GST-pull down experiments and conduct western blots to determine the results. By the end of this research, it will be determined if mutant Iqg1 alleles affect the binding of Iqg1 to Bnr1 and Bni1.

Biography

Caitlin Siehr is a junior at Missouri University of Science and Technology, pursuing a B.S. in Biological Sciences. She has studied under Dr. Shannon since September 2014. She is the President of Sigma Tau Delta, Missouri S&T’s English Honor Society, and enjoys taking care of her many plants and animals. After obtaining her Bachelor’s degree, she plans to further her education with graduate studies.

Research Category

Sciences

Presentation Type

Poster Presentation

Document Type

Poster

Location

Upper Atrium/Hall

Start Date

4-11-2017 9:00 AM

End Date

4-11-2017 11:45 AM

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Apr 11th, 9:00 AM Apr 11th, 11:45 AM

Cytokinesis and the effect of mutations on protein-protein interactions

Upper Atrium/Hall

Iqg1 is a protein involved in cytokinesis in budding yeast. It is required for the assembly and contraction of the actin ring- which is responsible for dividing the two cells. In previous studies, Iqg1 has been shown to interact with the two formin proteins Bni1 and Bnr1. The overall objective of my research is to determine how mutations affecting Iqg1 phosphorylation alter protein-protein interactions. My research began with a preparation of yeast extracts from three different strains- a wild type, a mutant that prevents Iqg1 phosphorylation, and a mutant that mimics Iqg1 phosphorylation. I then used these extracts to perform GST-pull down experiments and conduct western blots to determine the results. By the end of this research, it will be determined if mutant Iqg1 alleles affect the binding of Iqg1 to Bnr1 and Bni1.