Title

Investigating the function of the IQG1 domain in yeast cytokinesis

Presenter Information

Lindsey Pratt

Department

Biological Sciences

Major

Biological Sciences

Research Advisor

Shannon, Katie

Advisor's Department

Biological Sciences

Funding Source

Opportunities for Undergraduate Research Experiences (OURE)

Abstract

The budding yeast protein IQG1 is required for both actomyosin ring assembly and contraction during cytokinesis. Iqg1 interacts with two other proteins required for actin ring formation, the formins Bni1 and Bnr1. In order to further investigate the function of the interaction between Iqg1 and the formins, the first step is to identify which Iqg1 protein domain is required for the interaction. My project will be to investigate the binding using mutant strains lacking different Iqg1 domains. I will be preparing yeast protein extracts and performing protein binding analysis using GST pull down assays and Western blotting. Once the functional domain of Iqg1 is identified, we can further investigate the role of the Iqg1-formin interaction by doing a phenotypic analysis of Iqg1 mutants that cannot bind to formins.

Biography

Lindsey Pratt is a junior here at Missouri University of Science and Technology. She is majoring in biological sciences and minoring in chemistry. Lindsey belongs to several organizations including; Scrubs, National Society of Leadership and Success and the Honor Society. She works part time as a scribe for PhysAssist Scribes. She lives on a large cattle farm and enjoys spending most of her days outside. She hopes to be accepted to Mizzou’s medical program so that she can return home and practice as a general physician.

Research Category

Research Proposals

Presentation Type

Poster Presentation

Document Type

Poster

Location

Upper Atrium/Hallway

Presentation Date

11 Apr 2016, 9:00 am - 11:45 am

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Apr 11th, 9:00 AM Apr 11th, 11:45 AM

Investigating the function of the IQG1 domain in yeast cytokinesis

Upper Atrium/Hallway

The budding yeast protein IQG1 is required for both actomyosin ring assembly and contraction during cytokinesis. Iqg1 interacts with two other proteins required for actin ring formation, the formins Bni1 and Bnr1. In order to further investigate the function of the interaction between Iqg1 and the formins, the first step is to identify which Iqg1 protein domain is required for the interaction. My project will be to investigate the binding using mutant strains lacking different Iqg1 domains. I will be preparing yeast protein extracts and performing protein binding analysis using GST pull down assays and Western blotting. Once the functional domain of Iqg1 is identified, we can further investigate the role of the Iqg1-formin interaction by doing a phenotypic analysis of Iqg1 mutants that cannot bind to formins.