The Under-investigated Antioxidant Potential of Thiolactic Acid
Department
Chemistry
Major
Chemistry
Research Advisor
Ercal, Nuran
Advisor's Department
Chemistry
Funding Source
Opportunities for Undergraduate Research Experiences (OURE)
Abstract
Free radicals have numerous physiological functions within the cell, ranging from cellular signaling to the immune response. However, a surge in free radicals has the potential to devastate biological macromolecules, structures, and cellular function. Current research aims to identify the mechanism, effects, and elimination of free radicals through the action of antioxidant compounds, such as vitamin C. In light of this trend, the antioxidant potential of a common industrial thiol, known as thiolactic acid (TLA), was investigated. TLA’s scavenging capability was analyzed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and compared with known antioxidants, such as butylated hydroxytoluene (BHT), N-acetylcysteine (NAC), N-acetylcysteine amide (NACA), and uric acid, at various concentrations (200 μM-12.5 μM). The percent DPPH scavenged over time was plotted and the inhibitory concentration (IC50) was calculated for each compound. TLA demonstrated the fastest and greatest overall scavenging of DPPH radicals among the antioxidants tested.
Biography
Jonathan Kopel is a senior undergraduate student at Missouri University of Science studying biochemistry. He has worked in Dr. Nuran Ercal’s research laboratory investigating oxidative stress and antioxidant parameters of different compounds. In addition, Jonathan worked as intern at the Edward Doisy Research Center under the direction of Dr. Cho.
Research Category
Research Proposals
Presentation Type
Poster Presentation
Document Type
Poster
Location
Upper Atrium/Hallway
Presentation Date
11 Apr 2016, 9:00 am - 11:45 am
The Under-investigated Antioxidant Potential of Thiolactic Acid
Upper Atrium/Hallway
Free radicals have numerous physiological functions within the cell, ranging from cellular signaling to the immune response. However, a surge in free radicals has the potential to devastate biological macromolecules, structures, and cellular function. Current research aims to identify the mechanism, effects, and elimination of free radicals through the action of antioxidant compounds, such as vitamin C. In light of this trend, the antioxidant potential of a common industrial thiol, known as thiolactic acid (TLA), was investigated. TLA’s scavenging capability was analyzed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and compared with known antioxidants, such as butylated hydroxytoluene (BHT), N-acetylcysteine (NAC), N-acetylcysteine amide (NACA), and uric acid, at various concentrations (200 μM-12.5 μM). The percent DPPH scavenged over time was plotted and the inhibitory concentration (IC50) was calculated for each compound. TLA demonstrated the fastest and greatest overall scavenging of DPPH radicals among the antioxidants tested.
