Title

Comparing Phenotypes of IQG1 Mutants

Presenter Information

Madison Mara

Department

Biological Sciences

Major

Biological Sciences

Research Advisor

Shannon, Katie

Advisor's Department

Biological Sciences

Funding Source

Opportunities for Undergraduate Research Experiences (OURE)

Abstract

During cytokinesis, many mutations can arise that affect formation of the actomyosin ring. Iqg1 is a protein that is required for assembly and contraction of the actomyosin ring in budding yeast. This project is designed to compare the phenotypes of three separate IQG1 mutant alleles to examine any problems that arise during cytokinesis. In this research, a mutant (3A) that has three serines dephosphorylated via CDC14 mutated to alanine is compared to a mutant (4A) that has three serines and one threonine mutated to alanine and a mutant (3T) that has 3 serines mutated to theronines. This 3T mutant is able to be phosphorylated but it unable to be dephosphorylated. The goal in doing this is to compare cytokinesis defects in the 3A mutant, 4A mutant, and 3T mutant and see if threonine has a unique function and determine the phenotype of permanent phosphorylation. To confirm mutant phenotype, morphological analysis will be performed via microscopy and immunofluorescence to indicate if actin ring formation has been disrupted.

Biography

Madison is a Junior at Missouri University of Science and Technology pursuing a degree in Biological Sciences with an emphasis in pre medicine. She is the Vice President of Scrubs Pre-Med Club, the Director of Philanthropy for Zeta Tau Alpha, and has been conducting research in Dr. Shannon’s cytokinesis lab for two years. She plans on going to medical school once she graduates from Missouri S&T.

Research Category

Sciences

Presentation Type

Oral Presentation

Document Type

Presentation

Award

Sciences oral presentation, First place

Location

Ozark Room

Presentation Date

11 Apr 2016, 10:00 am - 10:30 am

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Apr 11th, 10:00 AM Apr 11th, 10:30 AM

Comparing Phenotypes of IQG1 Mutants

Ozark Room

During cytokinesis, many mutations can arise that affect formation of the actomyosin ring. Iqg1 is a protein that is required for assembly and contraction of the actomyosin ring in budding yeast. This project is designed to compare the phenotypes of three separate IQG1 mutant alleles to examine any problems that arise during cytokinesis. In this research, a mutant (3A) that has three serines dephosphorylated via CDC14 mutated to alanine is compared to a mutant (4A) that has three serines and one threonine mutated to alanine and a mutant (3T) that has 3 serines mutated to theronines. This 3T mutant is able to be phosphorylated but it unable to be dephosphorylated. The goal in doing this is to compare cytokinesis defects in the 3A mutant, 4A mutant, and 3T mutant and see if threonine has a unique function and determine the phenotype of permanent phosphorylation. To confirm mutant phenotype, morphological analysis will be performed via microscopy and immunofluorescence to indicate if actin ring formation has been disrupted.